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KMID : 1138520120150010007
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Journal of Pharmacopuncture
2012 Volume.15 No. 1 p.7 ~ p.11
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Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide induced Inflammatory Cytokine Expression in RAW 264.7 cells
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:Choi Eun-A
:Yoon Jeung-Won/:Choi Hak-Joo/:Kim Dong-Hee/:Yoo Hwa-Seung
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Abstract
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Aim: Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we investigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
Methods: RAW 264.7 cells cultured with LPS and various concentrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs).
Results: IS4 concentration inhibited the production of interleukin-1beta (IL-1β), interleukin-6 (IL-6), and granulocyte -macrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50㎍/ml) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF?α) stimulated by LPS.
Conclusion: IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.
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KEYWORD
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cytokine, GM-CSF, IL-1β, IL-6, lipopolysaccharide, TNF?α
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